Application

ExtrAvidin^®-Cy3^™ is suitable for the following applications: Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100Immunocytochemistry.Used for labeling.

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin^® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin^® is covalently conjugated to Cy3 dye and is used for various techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Cy3 is a trademark of Cytiva

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein